[Immunofluorescent diagnosis of rota-, corona- and pestivirus infections in calves].
Identifieur interne : 001633 ( Main/Exploration ); précédent : 001632; suivant : 001634[Immunofluorescent diagnosis of rota-, corona- and pestivirus infections in calves].
Auteurs : B. Mitov ; Kh E. Kharalambiev ; R. Bostandzhieva ; P. Tsvetkov ; K. PetkovaSource :
- Veterinarno-meditsinski nauki [ 0324-1068 ] ; 1984.
Descripteurs français
- KwdFr :
- Animaux, Bovins, Diarrhée virale bovine-maladie des muqueuses (diagnostic), Entérite (diagnostic), Entérite (médecine vétérinaire), Infections à Coronaviridae (diagnostic), Infections à Coronaviridae (médecine vétérinaire), Infections à Togaviridae (diagnostic), Infections à Togaviridae (médecine vétérinaire), Infections à rotavirus (diagnostic), Infections à rotavirus (médecine vétérinaire), Maladies des bovins (diagnostic), Pestivirus, Technique d'immunofluorescence, Tests sérologiques (médecine vétérinaire).
- MESH :
- diagnostic : Diarrhée virale bovine-maladie des muqueuses, Entérite, Infections à Coronaviridae, Infections à Togaviridae, Infections à rotavirus, Maladies des bovins.
- médecine vétérinaire : Entérite, Infections à Coronaviridae, Infections à Togaviridae, Infections à rotavirus, Tests sérologiques.
- Animaux, Bovins, Pestivirus, Technique d'immunofluorescence.
English descriptors
- KwdEn :
- Animals, Bovine Virus Diarrhea-Mucosal Disease (diagnosis), Cattle, Cattle Diseases (diagnosis), Coronaviridae Infections (diagnosis), Coronaviridae Infections (veterinary), Enteritis (diagnosis), Enteritis (veterinary), Fluorescent Antibody Technique, Pestivirus, Rotavirus Infections (diagnosis), Rotavirus Infections (veterinary), Serologic Tests (veterinary), Togaviridae Infections (diagnosis), Togaviridae Infections (veterinary).
- MESH :
- diagnosis : Bovine Virus Diarrhea-Mucosal Disease, Cattle Diseases, Coronaviridae Infections, Enteritis, Rotavirus Infections, Togaviridae Infections.
- veterinary : Coronaviridae Infections, Enteritis, Rotavirus Infections, Serologic Tests, Togaviridae Infections.
- Animals, Cattle, Fluorescent Antibody Technique, Pestivirus.
Abstract
Bovine hyperimmune fluorescent sera were obtained for indication of the rota- and corona-virus infections in calves, which were highly specific and of immunofluorescent titers within the 1:32-64 range. Their testing was carried out via the direct immunofluorescence method, using histologic cross-sectioned material and impression preparations of the mucous membrane of calves died of enteritis as well as cell cultures of fetal calf kidney and organic cultures of calf trachea all contaminated, resp., infected with feces. The study of the intestinal membrane of a total of 100 diseased calves, employing this method, has demonstrated a rotavirus antigen in 38.0 per cent of the cases, and a coronavirus one--in 37.0 per cent. The causative agent of mucous disease (a pestivirus) was demonstrated by means of the respective conjugate in 47.3 per cent of the cases, using abomasal membrane of a total of 55 calves. Rota- and corona-virus infections were also established in the abomasal membrane of diseased calves, and pesti-virus infections--in the intestinal mucosa as well. Established were also mixed infections with the three viruses taking part at various combinations. A celloidin method was worked out for making immunofluorescent preparations of infected monolayer cell cultures. In the cell cultures infected with feces the totavirus antigen was demonstrated in 19.4 per cent of the cases, and the coronavirus one--in 25.6 per cent.
PubMed: 6098987
Affiliations:
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Le document en format XML
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Mitov</name></author><author><name sortKey="Kharalambiev, Kh E" sort="Kharalambiev, Kh E" uniqKey="Kharalambiev K" first="Kh E" last="Kharalambiev">Kh E. Kharalambiev</name></author><author><name sortKey="Bostandzhieva, R" sort="Bostandzhieva, R" uniqKey="Bostandzhieva R" first="R" last="Bostandzhieva">R. Bostandzhieva</name></author><author><name sortKey="Tsvetkov, P" sort="Tsvetkov, P" uniqKey="Tsvetkov P" first="P" last="Tsvetkov">P. Tsvetkov</name></author><author><name sortKey="Petkova, K" sort="Petkova, K" uniqKey="Petkova K" first="K" last="Petkova">K. Petkova</name></author></analytic><series><title level="j">Veterinarno-meditsinski nauki</title><idno type="ISSN">0324-1068</idno><imprint><date when="1984" type="published">1984</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bovine Virus Diarrhea-Mucosal Disease (diagnosis)</term><term>Cattle</term><term>Cattle Diseases (diagnosis)</term><term>Coronaviridae Infections (diagnosis)</term><term>Coronaviridae Infections (veterinary)</term><term>Enteritis (diagnosis)</term><term>Enteritis (veterinary)</term><term>Fluorescent Antibody Technique</term><term>Pestivirus</term><term>Rotavirus Infections (diagnosis)</term><term>Rotavirus Infections (veterinary)</term><term>Serologic Tests (veterinary)</term><term>Togaviridae Infections (diagnosis)</term><term>Togaviridae Infections (veterinary)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Diarrhée virale bovine-maladie des muqueuses (diagnostic)</term><term>Entérite (diagnostic)</term><term>Entérite (médecine vétérinaire)</term><term>Infections à Coronaviridae (diagnostic)</term><term>Infections à Coronaviridae (médecine vétérinaire)</term><term>Infections à Togaviridae (diagnostic)</term><term>Infections à Togaviridae (médecine vétérinaire)</term><term>Infections à rotavirus (diagnostic)</term><term>Infections à rotavirus (médecine vétérinaire)</term><term>Maladies des bovins (diagnostic)</term><term>Pestivirus</term><term>Technique d'immunofluorescence</term><term>Tests sérologiques (médecine vétérinaire)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Bovine Virus Diarrhea-Mucosal Disease</term><term>Cattle Diseases</term><term>Coronaviridae Infections</term><term>Enteritis</term><term>Rotavirus Infections</term><term>Togaviridae Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Diarrhée virale bovine-maladie des muqueuses</term><term>Entérite</term><term>Infections à Coronaviridae</term><term>Infections à Togaviridae</term><term>Infections à rotavirus</term><term>Maladies des bovins</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Entérite</term><term>Infections à Coronaviridae</term><term>Infections à Togaviridae</term><term>Infections à rotavirus</term><term>Tests sérologiques</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronaviridae Infections</term><term>Enteritis</term><term>Rotavirus Infections</term><term>Serologic Tests</term><term>Togaviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Fluorescent Antibody Technique</term><term>Pestivirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Pestivirus</term><term>Technique d'immunofluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bovine hyperimmune fluorescent sera were obtained for indication of the rota- and corona-virus infections in calves, which were highly specific and of immunofluorescent titers within the 1:32-64 range. Their testing was carried out via the direct immunofluorescence method, using histologic cross-sectioned material and impression preparations of the mucous membrane of calves died of enteritis as well as cell cultures of fetal calf kidney and organic cultures of calf trachea all contaminated, resp., infected with feces. The study of the intestinal membrane of a total of 100 diseased calves, employing this method, has demonstrated a rotavirus antigen in 38.0 per cent of the cases, and a coronavirus one--in 37.0 per cent. The causative agent of mucous disease (a pestivirus) was demonstrated by means of the respective conjugate in 47.3 per cent of the cases, using abomasal membrane of a total of 55 calves. Rota- and corona-virus infections were also established in the abomasal membrane of diseased calves, and pesti-virus infections--in the intestinal mucosa as well. Established were also mixed infections with the three viruses taking part at various combinations. A celloidin method was worked out for making immunofluorescent preparations of infected monolayer cell cultures. In the cell cultures infected with feces the totavirus antigen was demonstrated in 19.4 per cent of the cases, and the coronavirus one--in 25.6 per cent.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bostandzhieva, R" sort="Bostandzhieva, R" uniqKey="Bostandzhieva R" first="R" last="Bostandzhieva">R. Bostandzhieva</name><name sortKey="Kharalambiev, Kh E" sort="Kharalambiev, Kh E" uniqKey="Kharalambiev K" first="Kh E" last="Kharalambiev">Kh E. Kharalambiev</name><name sortKey="Mitov, B" sort="Mitov, B" uniqKey="Mitov B" first="B" last="Mitov">B. Mitov</name><name sortKey="Petkova, K" sort="Petkova, K" uniqKey="Petkova K" first="K" last="Petkova">K. Petkova</name><name sortKey="Tsvetkov, P" sort="Tsvetkov, P" uniqKey="Tsvetkov P" first="P" last="Tsvetkov">P. Tsvetkov</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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